P r o g r a m s -
 

Workshop 1

Workshop 2

Symposium

Manufacturers session

Poster list

 

 
   

The main symposium, featuring lectures and posters, will provide a real overview of the most recent advances in all the application fields where TLC has a place. Training and the manufacturers’ sessions will be held in conjunction with the main symposium but functioning as a separate program.


The training program is strongly focused to provide all your needs in a short time. This time a special workshop will be targeted on validation. The best experts will be there to guide you through each step and explain what you need to know, independent of your level of expertise.


Such an event wouldn’t be possible without support from the manufacturers. As in all other high technology fields, they are at the forefront of innovation and technical improvement. Their collaboration with world leaders in the field from the early days of TLC has ensured development of Instrumental HPTLC to a modern analytical technique.


We are looking forward to share our knowledge, ideas and enthusiasm!

 

 

 

Monday, October 9, 2006

11:00 - 14:00          Registration for the Symposium   

14:00 : Workshop 1 - HPTLC Steps from Application to Quantification 

The state of the art of HPTLC employs automatic systems for application, development, detection and documentation confirming high reliability and sensitivity. High performance and resolution obtained with the modern technique delivers suitable solutions to your tasks. The training program is strongly focused to provide the most important needs and current trends, like HPTLC-MS, in a short time.

  • Pretreatment of the plate, sample application

  • Simple approach for solvent optimization: selectivity first

  • How to control the chamber climate?

  • AMD: a dream of a separation, but how to get it?

15:30 : Break

16:00 : Workshop continuation 

  • How to visualize substances on a plate – benefits of multiple detection

  • Chemical and biological detection: impressive tools of HPTLC

  • Densitometric techniques: promising and limiting aspects

  • Confirmation of results by mass spectrometry

18:00 : End

Dr. Gerda Morlock
University of Hohenheim
Institute of Food Chemistry
Garbenstrasse 28
D-70599 Stuttgart
Tel +49-(0)711-459-4092
Fax +49-(0)711-459-4096
gmorlock@uni-hohenheim.de

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Monday, October 9, 2006

14:00 : Workshop 2 - HPTLC Method validation

Method validation is the process of proving that an analytical method is acceptable for its intended purpose. In general, methods for regulatory submission must include studies on specificity, selectivity, analytical response, accuracy, precision, range, detection limit, quantification limit, and robustness. Doing a thorough method validation can be tedious, but the consequences of not doing it right are wasted time, money, and resources.

  • Definitions: validation, qualification, calibration 

  • Validation parameters: analytical response, accuracy, trueness, precision, robustness, ruggedness, LOD, LOQ according to ISO 11843 (2005), specificity, selectivity, overall relative and maximum uncertainty

  • Statistical analysis of results

15:30 : Break

16:00 : Workshop continuation

  • Definition of uncertainty

  • Analytical measurement and uncertainty

  • Modes of determination and calculation of uncertainty

18:00 : End

 

Prof. Dr. I. Schellenberg

Anhalt University of Applied Sciences Center of Life Sciences

 Strenzfelder Allee 28

D-06406 Bernburg

Germany

Phone: +49(0) 3471/355-1188

Fax:     +49(0) 3471/355-1189

schellenberg@loel.hs-anhalt.de

                       

 18:00 - 19:00          Welcome cocktail  

 (Evening free)

 

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Symposium

Tuesday, October 10, 2006                                

8:30      Welcome by L. Kroh, Berlin/D

News, fundamentals and theoretical aspects

Chair:   F. Geiss,  / It, R. Kaisen/ De

8:40     From modelling separations to estimation of biopartitioning properties: use of the solvatation parameter model in thin-layer chromatography’

C. F. Poole, Wayne State University, Detroit, Michigan, USA     

 

9:00     Variables influencing separation efficiency in Pressurized Planar Electrochromatography

T. H. Dzido, Medical University of Lublin, Pl    

 

9:20     Application of subambient and elevated temperatures for TLC separation and detection protocols

P. K. Zarzycki, Laboratory of Toxicology, Koszalin, Pl    

 

9:40     ‘Printing’ for derivatization in HPTLC

W. Schwack, University of Hohenheim, Stuttgart, De  

 

10:00   Fluorescence spectroscopy in planar chromatography

B. Spangenberg, University of Offenburg, De  

 

10:20  Coffee break (posters and exhibition)

 

 

Specific applications, where HPTLC is the method of choice, benchmarking studies

 

A - Food analysis

 

Chairs:  L. Kroh / D, W. Schwack / De

 

10:50   HPTLC for food analysis in India

P. K.  Jaiswal, Central Agmark Laboratory, Maharashtra, In 

 

11:10   Quantitative TLC in characterisation of components in food

M. Prosek, National Institute of Chemistry, Ljubljana, Sl  

 

11:30   Histamine in fish & theanine in tea, two cases for Planar Chromatography’

K. Speer, Technical University Dresden, De  

 

11:50   HPTLC as the choice tool for quality control in Chilean salmon culture industry
            M. Vega, University of Concepcion, Ci  

 

12:10   Qualitative and quantitative determination of phenolic acids during commercial potato processing: AMD-HPTLC as powerful tool

J. Mäder, Technical University Berlin, De 

 

12:30  Lunch

 

B - Plants analysis

 

Chairs: I. Schellenberg / D, H. Vuorela / Fi

 

14:00   Thin-layer chromatography of lipid fractions from tree nut species

G. Pujol, University Rovira i Virgili, Tarragona, Sp  

 

14:20   Natural products with chromatographic challenges-ceramides and calystegines

A. Opitz, University of Halle, De 

 

14:40   HPTLC Fingerprinting of A. Iongifolia Nees, W. Somnifera and C. Officinalis
R. S. Pawar, Dr. H.S. Gour University, Sagar, In  

 

15:00   Rapid detection of residues of cardenolides of Nerium Oleander (linn.) by high-performance thin-layer chromatography (HPTLC) in autopsied samples

B.M. Mohan, Forensic Science Laboratories, Bangalore, In 

 

15:20   Standardized HPTLC methods for identification of botanicals and detection of adulteration
E. Reich, CAMAG Laboratory, Muttenz, Ch 

 

15:40  Coffee break (posters and exhibition)

 

 

C - Clinical applications

Chairs: J. Muething / De, I. Ojanperae / Fi

 

16:40   Degradation product dosage in dermatological products - HPTLC Method

C. Pettiti, International Technical Center, Bayer Healthcare, Gaillard, Fr  

 

17:00   Comparative studies on analyses of total human epidermal lipids by HPTLC with and without prior
           separation of lipid classes on LC-NH2 columns.

 Iuliana Popa, University of Lyon I, Laboratory of Dermatological Research, Lyon, Fr 

 

 

17:20  HPTLC for the analysis of protein digests and peptides

M. Schulz, Merck, Darmstadt, De 

 

17:45   End

 

19:30   Start from Hotel SI by bus to the Cruising Dinner
 

20:30   Dinner on board
 

24:00   Return to Hotel SI


 

Wednesday, October 11, 2006             

Strong features of HPTLC

 

Chairs:   P. Bernard Savary / Fr, G. Morlock / De

 

8:30     Use of HPTLC as an orthogonal analytical method for determining active pharmaceutical ingredients at production sites

 L. Vicard, LAPS, Sanofi-Aventis, Neuville-sur-Saône, Fr  

 

8:50     HPTLC as a useful method in a laboratory for preparative chromatography

D. Dron, Laboratoire Preparative, Oril, Bolbec, Fr 

 

9:10     A general HPTLC detection of analytes, including non-fluorescent ones, by changes in fluorescent emission of the coralyne cation

V. L. Cebolla, CSIC Instituto de Carboquimica, Zaragoza, Sp 

 

9:30     Poster presentations

 

9:50     Poster award by L. Kroh / De

 

10:00  Coffee break (posters and exhibition)

 

 

Bioactivity tests, specific detection and coupling methods

 

Chairs:   B. Spangenberg / D, M. Aranda / Cl

 

10:50   Bioluminescence for TLC detection

W. Kreiss, Bayer Industry Services Analytics, Leverkusen, De  

 

11:10   Characterisation of natural waters using HPTLC and toxicity-directed analysis

W. Seitz, Zweckverband der Landeswasserversorgung, Langenau, De 

 

11:30   Bioresponse-linked instrumental analysis: Bridging the gap between cause and effect? Risk analysis – Risk assessment: Detection of bio-effect environmental compounds by bio-activity based analysis in HPTLC.
C. Weins, Department of Chemistry, organic analytical chemistry, University of Basel, Ch 

 

11:50   Combination of high-performance thin-layer chromatography immunostaining and mass spectrometry: clinical applications to the structural characterization of potential glycosphingolipid targets for anti-cancer therapies

J. Müthing, Medical University of Münster, De  

 

12:10  Coupling of planar chromatography with mass spectrometry -
comparison of three approaches (DART, ESI and APGD)

 G. Morlock, University of Hohenheim, Stuttgart, De 

 

12:30  Lunch


 

Manufacturers session

 

Chair:   C.F.Poole / USA

 

14:00    AccuTOF-DART: a new technology for fast screening and characterization

            Bob Hertens, Jeol, Zaventem, Belgium  

               

 

14:20   Improving HPTLC results by means of standardization & new Instruments

           Matthias Loppacher, Camag, Muttenz,Switzerland  

               

 

14:40   Radioactivity detection in HPTLC

           Günter Dietzel, Raytest, Straubenhardt,Germany 

 

15:00   Coffee break

               

15:20   Tradition oblige - Committed to Thin Layer Chromatography

           Dagmar Leiss, E. Merck, Darmstadt, Germany  

 

 

15:40   Rapid Screening of Complex Mixtures by TLC-Bioluminescence

           Sheryl Verbitski, ChromaDex Analytics, Boulder, CO, USA   


16:00   New book: High-Performance Thin-Layer Chromatography

           for the analysis of Medical Plants

           Heinz-Emil Hauck, E. Merck, Darmstadt, Germany

 

16:10   Lyon, Berlin, and what’s next?

           Pierre Bernard-Savary, Chromacim, France

 

16:20   Closing remarks by C.F.Poole,

 

17:00   End of symposium

 

Poster list

 

Salmon feed quality control by planar chromatography.Mario Aranda

  

Artemisinin quantification in plant material by PTLC using a DIONEX Accelerated Solvent Extractor (ASE®) device and Artemether as internal standard.A. Benakis, X. Simonnet, M. Quennoz, R. Locher 

 

Camera-less TLC.V.G.Berezkin

  

New method and device for feeding TLC plates with mobile phase.V.G. Berezkin, A.V. Chausov

  

Repetition of the first work on thin-layer chromatography.V.G.Berezkin, I.I.Ashrapova

  

Two-dimensional chromatography on reducing plate as express method of planar chromatography
V.G.Berezkin, V.F.Tret’yakov, Aliyu Auwal, G.A.Nekhoroshev, E.V.Kormishkina

  

Comparison between TLC and HPTLC of pharmaceutical relevant herbal drugs.T. Brem, S. Geiger, J. Heilmann und G. Franz

 

HPTLC as a useful tool for determining lipid composition of Archaeosomes, a new type of nanocarrier for gene and drug delivery. Sandrine Cammas-Marion, Sandrine Autret, Loïc Lemiègre, Thierry Benvegnu

  

The monitoring of the wine origin by Reversed-Phase Thin-Layer Chromatography" Claudia Cimpoiu, Anamaria Hosu, Rodica Briciu, Vasile Miclaus

  

Rapid and Sensitive TLC-Densitometric Analysis of Artemisinin  in Artemisia annua L.Thongchai  Koobkokkruad, Araya  Chochai, Chalermpol  Kerdmanee,Wanchai  De-Eknamku 

 

Densitometric Determination of Ondansetron Impurity B in Pharmaceutical Formulation. P. Golja,

 

TLC analysis of pharmaceutical preparations containing vitamins A, D and E.Ana-Maria Hossu, Mihaela ILIE, Cristiana Raduslescu 

 

 Automated multiple development (AMD) and analysis of CD15 glycosphingolipids directly from high-performance thin-layer chromatography plates by infrared matrix-assisted laser desorption/ionization orthogonal time-of-flight mass spectrometry.Marcel Hülsewig, Klaus Dreisewerd, Stefan Berkenkamp, Jasna Peter-Katalinić, and Johannes Müthing 

 

 HPTLC as a tool to rapid assess the elicitor responsiveness of hairy roots cultured in the LiquidLabTM reactor
Medina-Bolivar Fabricio

  

SPE-HPTLC of Proanthocyanidins from The Bark of Different Species and Clones of Salix
Loretta Pobłocka-Olech, Mirosława Krauze-Baranowska

  

Plant Biomarker Pattern – Phytochemical Pattern in Apera spica-venti after exposure to six different herbicides using High Performance Thin-Layer Chromatography (HPTLC).Helle Weber Ravn, Lise Lauridsen, Per Nielsen Kudsk Solvejg Kopp Mathiassen & Maibritt Hjorth 

  

Plant Biomarker Pattern - High Performance Thin-Layer Chromatography (HPTLC) used to detect a phytochemical pattern in five different plant species after exposure to glyphosate.
Helle Weber Ravn, Lise Lauridsen, Per Nielsen Kudsk Solvejg Kopp Mathiassen & Maibritt Hjorth 

  

Evaluation of P. acnes lipase activity by HPTLC: Evidence of an antibacterial cream efficiency in a clinical study.
Ophélie Lejeune, Cécile Viodé, Daniel Redoulès 

  

Determination of Apigenin in Some Iranian Liquid Products of Matricaria Chamomilla by High Performance Thin-Layer Chromatography (HPTLC).Nazli Nader, Somayeh Esmaeili, Farzaneh Naghibi, Mahmoud Mosaddegh

 

TLC Seperation of Th(IV) and Ln(III) on Various Stationary Phases Using HDEHDTP as Complexing Agent
Maria – Loredana Soran2, Maria Curtui3, Alexandria Nan 

  

Identification of designer drugs with a multi-technique approach without reference standards. Ilkka Ojanperä 

  

OPLC: A rapid chromatographic method to research active components for the skin care from a polar natural ingredient. Virginie Pecher, Cécile Lamy, Sylvie Darnault, Patrice André, Patrick Chaimbault, Claire Elfakir

  

HPTLC for a best knowledge of Vineyard.Carole Pineda, Roger Cantagrel, Sylvie Darnault, Cécile Lamy

 Patrice André 

 

 AMD-HPTLC analysis of reaction products resulting from a thermal induced degradation of onion (Allium cepa) flavonols’.S. Rohn

  

Monitoring the thermal induced degradation of selected coumarin derivatives using AMD-HPTLC.S. Rohn

  

Qualification of luminecent inhibition on TLC Plates.W. Seitz 

  

 Application of Planar Chromatography on Determination of Sterigmatocystin in foods. I. Skarkova, V. Ostry, and I. Prochazkova

  

Development of HPTLC Method for Determination of Patulin in Apple Products.J. Skarkova, V. Ostry, and I. Prochazkova 

 

High-performance thin-layer chromatography immunodetection coupled with nanoelectrospray ionization quadrupole time-of-flight mass spectrometry for full structural characterization of influenza A virus binding isomeric neolacto-series gangliosides from human granulocytes
Jamal Souady, Gottfried Pohlentz, Iris Meisen, Jasna Peter-Katalinić, and Johannes Müthing

 

The Determination of Endo- and Exogenic Steroids in Biological Liquids by Thin-Layer Chromatography Methods
Kartsova L.A.*, Strelnikova E.G.

 

An HPTLC-OPLC approach for understanding the microbial metabolism in the presence of different compolex carbohydrates mixtures: The case of Bifidobacterium adolescentis MB 239.T. Bernardi, E. Tamburini, G. Vaccari 

 

Study On The HPTLC Fingerprints of Bupleurum spp.Mr. TIAN Run-tao, Dr.XIE Pei-shan 

  

Optimization of Conditions Affecting Direct Bioautography Assay for Antimicrobial Compounds
Päivi Oinonen, Karoliina Sainio, Aila Mettälä, Annele Hatakka, Heikki Vuorela , and Pia Vuorela
 

  

 “Rapid Screening of Complex Mixtures using TLC-Bioluminescence.” Sheryl M. Verbitski, Gerald T. Gourdin, Larissa M. Ikenouye, James D. McChesney

 

Authentication of Aucklandia lappa Decne. (Compositae) by Automated.Multiple Development System 2 (AMD 2)
Mr. Wang Xiang-hong, Dr.XIE Pei-shan

 

Application of thin layer chromatography to profiling 3,4-methylenedioxymethamphetamine (MDMA).Zakrzewska1, J. Kochana1, A. Parczewski1,2, 
 

Separation and detection of Ergosterol and related Stereoids using temperature controlled planar chromatography
P. K. Zarzycki, M. Baran, E. Wlodarczyk, T. Heese
 

Planar chromatography in European Pharmacopeia, P. K. Zarzycki, M. B. Zarzycka

Determination of sucralose in fruit jelly by planar chromatography. Funk, S., Morlock, G.

 

Optimization of the Immunodetection in planar chromatography.R. Akkad, G. Morlock, W. Schwack

 

New validated method for determination of acrylamide in potato chips by planar chromatography Müller, D.,

Morlock, G.

 

HPTLC/DART TOFT compared HPLC-ESI MS. G. Morlock 

  

 Possibilities and limitations of the employment of y-spectroscopy in planar  chromatography. Blagodarov, V., Morlock, G., Schwack, W

Efficiency of a new device for application of reagents onto HPTLC plates or foils. Stiefel, C., Morlock, G.,

 Schwack, W. 


Simultaneous determination of caffeine, ergotamine and metamizol in solid pharmaceutical formulation by HPTLC/UV/FLD and HPTLC/ESI-MS.M. Aranda, G. Morlock 

 

Thin-Layer Chromatography of lipid fraction in tree nuts species. Gerard Pujol,Josep M. Orellana, Nicolas Rozés, Joan R. Duran,Antoni Romeu. University Rovira i Virgili.43007 Tarragona Spain.

 

Quantification of pigments Astaxanthin and its Isomers by HPTLC in salmon fish feed . Vega H. Mario, Aranda B. Mario, Vega M. Mario, Capdeville Claudio, Villegas Ricardo, Muñoz Katherine, Bastías Corina and Torres Olga 

 

HPTLC as Tool for Quality control in salmon culture. Evaluation of nutritive quality in salmon feed.Vega H. Mario, Aranda B. Mario, Vega M. Mario, Capdeville Claudio,Villegas Ricardo, Muñoz Katherine, Bastías Corina and

Torres Olga 

 

Simultaneous Determination of vitamin B2, vitamin B6, Niacin, caffein and Taurin by HPTLC/UV/FLD and HPTLC/ES-MS. M. Aranda, G. Morlock 

 

 

 

 

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