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11:00 - 14:00
Registration for the Symposium 14:00 : Workshop 1 - HPTLC Steps from Application to Quantification The state of the art of HPTLC employs automatic systems for application, development, detection and documentation confirming high reliability and sensitivity. High performance and resolution obtained with the modern technique delivers suitable solutions to your tasks. The training program is strongly focused to provide the most important needs and current trends, like HPTLC-MS, in a short time.
15:30 : Break
18:00 : End Dr. Gerda Morlock
14:00 : Workshop 2 - HPTLC Method validation Method validation is the process of proving that an analytical method is acceptable for its intended purpose. In general, methods for regulatory submission must include studies on specificity, selectivity, analytical response, accuracy, precision, range, detection limit, quantification limit, and robustness. Doing a thorough method validation can be tedious, but the consequences of not doing it right are wasted time, money, and resources.
15:30 : Break 16:00 : Workshop continuation
18:00 : End
Prof. Dr. I. Schellenberg Anhalt University of Applied Sciences Center of Life Sciences Strenzfelder Allee 28 D-06406 Bernburg Germany Phone: +49(0) 3471/355-1188 Fax: +49(0) 3471/355-1189 schellenberg@loel.hs-anhalt.de
18:00 - 19:00 Welcome cocktail (Evening free)
Tuesday, October 10, 2006 8:30 Welcome by L. Kroh, Berlin/D News, fundamentals and theoretical aspects Chair: F. Geiss, / It, R. Kaisen/ De 8:40 From modelling separations to estimation of biopartitioning properties: use of the solvatation parameter model in thin-layer chromatography’ C. F. Poole, Wayne State University, Detroit, Michigan, USA
9:00 Variables influencing separation efficiency in Pressurized Planar Electrochromatography T. H. Dzido, Medical University of Lublin, Pl
9:20 Application of subambient and elevated temperatures for TLC separation and detection protocols P. K. Zarzycki, Laboratory of Toxicology, Koszalin, Pl
9:40 ‘Printing’ for derivatization in HPTLC W. Schwack, University of Hohenheim, Stuttgart, De
10:00 Fluorescence spectroscopy in planar chromatography B. Spangenberg, University of Offenburg, De
10:20 Coffee break (posters and exhibition)
Specific applications, where HPTLC is the method of choice, benchmarking studies
A - Food analysis
Chairs: L. Kroh / D, W. Schwack / De
10:50 HPTLC for food analysis in India P. K. Jaiswal, Central Agmark Laboratory, Maharashtra, In
11:10 Quantitative TLC in characterisation of components in food M. Prosek, National Institute of Chemistry, Ljubljana, Sl
11:30 Histamine in fish & theanine in tea, two cases for Planar Chromatography’ K. Speer, Technical University Dresden, De
11:50 HPTLC as the choice tool for
quality control in Chilean salmon culture industry
12:10 Qualitative and quantitative determination of phenolic acids during commercial potato processing: AMD-HPTLC as powerful tool J. Mäder, Technical University Berlin, De
12:30 Lunch
B - Plants analysis
Chairs: I. Schellenberg / D, H. Vuorela / Fi
14:00 Thin-layer chromatography of lipid fractions from tree nut species G. Pujol, University Rovira i Virgili, Tarragona, Sp
14:20 Natural products with chromatographic challenges-ceramides and calystegines A. Opitz, University of Halle, De
14:40 HPTLC Fingerprinting of A.
Iongifolia Nees, W. Somnifera and C. Officinalis
B.M. Mohan, Forensic Science Laboratories, Bangalore, In
15:20 Standardized HPTLC methods
for identification of botanicals and detection of adulteration
15:40 Coffee break (posters and exhibition)
C - Clinical applications Chairs: J. Muething / De, I. Ojanperae / Fi
16:40 Degradation product dosage in dermatological products - HPTLC Method C. Pettiti, International Technical Center, Bayer Healthcare, Gaillard, Fr
17:00 Comparative studies
on analyses of total human epidermal lipids by HPTLC with and without
prior Iuliana Popa, University of Lyon I, Laboratory of Dermatological Research, Lyon, Fr
17:20 HPTLC for the analysis of protein digests and peptides M. Schulz, Merck, Darmstadt, De
17:45 End
19:30 Start from Hotel SI by bus to
the Cruising Dinner 20:30 Dinner on board 24:00 Return to Hotel SI
Wednesday, October 11, 2006 Strong features of HPTLC
Chairs: P. Bernard Savary / Fr, G. Morlock / De
L. Vicard, LAPS, Sanofi-Aventis, Neuville-sur-Saône, Fr
8:50 HPTLC as a useful method in a laboratory for preparative chromatography D. Dron, Laboratoire Preparative, Oril, Bolbec, Fr
V. L. Cebolla, CSIC Instituto de Carboquimica, Zaragoza, Sp
9:30 Poster presentations
9:50 Poster award by L. Kroh / De
10:00 Coffee break (posters and exhibition)
Bioactivity tests, specific detection and coupling methods
Chairs: B. Spangenberg / D, M. Aranda / Cl
10:50 Bioluminescence for TLC detection W. Kreiss, Bayer Industry Services Analytics, Leverkusen, De
11:10 Characterisation of natural waters using HPTLC and toxicity-directed analysis W. Seitz, Zweckverband der Landeswasserversorgung, Langenau, De
11:30 Bioresponse-linked
instrumental analysis: Bridging the gap between cause and effect? Risk
analysis – Risk assessment: Detection of bio-effect environmental
compounds by bio-activity based analysis in HPTLC.
J. Müthing, Medical University of Münster, De
G. Morlock, University of Hohenheim, Stuttgart, De
12:30 Lunch
Chair: C.F.Poole / USA
14:00 AccuTOF-DART: a new technology for fast screening and characterization Bob Hertens, Jeol, Zaventem, Belgium
14:20 Improving HPTLC results by means of standardization & new Instruments Matthias Loppacher, Camag, Muttenz,Switzerland
14:40 Radioactivity detection in HPTLC Günter Dietzel, Raytest, Straubenhardt,Germany
15:00 Coffee break Dagmar Leiss, E. Merck, Darmstadt, Germany
15:40 Rapid Screening of Complex Mixtures by TLC-Bioluminescence Sheryl Verbitski, ChromaDex Analytics, Boulder, CO, USA
for the analysis of Medical Plants Heinz-Emil Hauck, E. Merck, Darmstadt, Germany
16:10 Lyon, Berlin, and what’s next? Pierre Bernard-Savary, Chromacim, France
16:20 Closing remarks by C.F.Poole,
17:00 End of symposium
Salmon feed quality control by planar chromatography.Mario Aranda
Artemisinin quantification in plant material by PTLC using a DIONEX Accelerated Solvent Extractor (ASE®) device and Artemether as internal standard.A. Benakis, X. Simonnet, M. Quennoz, R. Locher
Camera-less TLC.V.G.Berezkin
New method and device for feeding TLC plates with mobile phase.V.G. Berezkin, A.V. Chausov
Repetition of the first work on thin-layer chromatography.V.G.Berezkin, I.I.Ashrapova
Two-dimensional chromatography on
reducing plate as express method of planar chromatography
Comparison between TLC and HPTLC of pharmaceutical relevant herbal drugs.T. Brem, S. Geiger, J. Heilmann und G. Franz
HPTLC as a useful tool for determining lipid composition of Archaeosomes, a new type of nanocarrier for gene and drug delivery. Sandrine Cammas-Marion, Sandrine Autret, Loïc Lemiègre, Thierry Benvegnu
The monitoring of the wine origin by Reversed-Phase Thin-Layer Chromatography" Claudia Cimpoiu, Anamaria Hosu, Rodica Briciu, Vasile Miclaus
Rapid and Sensitive TLC-Densitometric Analysis of Artemisinin in Artemisia annua L.Thongchai Koobkokkruad, Araya Chochai, Chalermpol Kerdmanee,Wanchai De-Eknamku
Densitometric Determination of Ondansetron Impurity B in Pharmaceutical Formulation. P. Golja,
TLC analysis of pharmaceutical preparations containing vitamins A, D and E.Ana-Maria Hossu, Mihaela ILIE, Cristiana Raduslescu
Automated multiple development (AMD) and analysis of CD15 glycosphingolipids directly from high-performance thin-layer chromatography plates by infrared matrix-assisted laser desorption/ionization orthogonal time-of-flight mass spectrometry.Marcel Hülsewig, Klaus Dreisewerd, Stefan Berkenkamp, Jasna Peter-Katalinić, and Johannes Müthing
HPTLC as a tool to rapid assess
the elicitor responsiveness of hairy roots cultured in the LiquidLabTM
reactor
SPE-HPTLC of Proanthocyanidins
from The Bark of Different Species and Clones of Salix
Plant Biomarker Pattern – Phytochemical Pattern in Apera spica-venti after exposure to six different herbicides using High Performance Thin-Layer Chromatography (HPTLC).Helle Weber Ravn, Lise Lauridsen, Per Nielsen Kudsk Solvejg Kopp Mathiassen & Maibritt Hjorth
Plant Biomarker Pattern - High
Performance Thin-Layer Chromatography (HPTLC) used to detect a
phytochemical pattern in five different plant species after exposure to
glyphosate.
Evaluation of P. acnes lipase
activity by HPTLC: Evidence of an antibacterial cream efficiency in a
clinical study.
Determination of Apigenin in Some Iranian Liquid Products of Matricaria Chamomilla by High Performance Thin-Layer Chromatography (HPTLC).Nazli Nader, Somayeh Esmaeili, Farzaneh Naghibi, Mahmoud Mosaddegh
TLC Seperation of Th(IV) and
Ln(III) on Various Stationary Phases Using HDEHDTP as Complexing Agent
Identification of designer drugs with a multi-technique approach without reference standards. Ilkka Ojanperä
OPLC: A rapid chromatographic method to research active components for the skin care from a polar natural ingredient. Virginie Pecher, Cécile Lamy, Sylvie Darnault, Patrice André, Patrick Chaimbault, Claire Elfakir
HPTLC for a best knowledge of Vineyard.Carole Pineda, Roger Cantagrel, Sylvie Darnault, Cécile Lamy
AMD-HPTLC analysis of reaction products resulting from a thermal induced degradation of onion (Allium cepa) flavonols’.S. Rohn
Monitoring the thermal induced degradation of selected coumarin derivatives using AMD-HPTLC.S. Rohn
Qualification of luminecent inhibition on TLC Plates.W. Seitz
Application of Planar Chromatography on Determination of Sterigmatocystin in foods. I. Skarkova, V. Ostry, and I. Prochazkova
Development of HPTLC Method for Determination of Patulin in Apple Products.J. Skarkova, V. Ostry, and I. Prochazkova
High-performance thin-layer
chromatography immunodetection coupled with nanoelectrospray ionization
quadrupole time-of-flight mass spectrometry for full structural
characterization of influenza A virus binding isomeric neolacto-series
gangliosides from human granulocytes
The Determination of Endo- and
Exogenic Steroids in Biological Liquids by Thin-Layer Chromatography
Methods
An HPTLC-OPLC approach for understanding the microbial metabolism in the presence of different compolex carbohydrates mixtures: The case of Bifidobacterium adolescentis MB 239.T. Bernardi, E. Tamburini, G. Vaccari
Study On The HPTLC Fingerprints of Bupleurum spp.Mr. TIAN Run-tao, Dr.XIE Pei-shan
Optimization of Conditions
Affecting Direct Bioautography Assay for Antimicrobial Compounds
“Rapid Screening of Complex Mixtures using TLC-Bioluminescence.” Sheryl M. Verbitski, Gerald T. Gourdin, Larissa M. Ikenouye, James D. McChesney
Authentication of Aucklandia
lappa Decne. (Compositae) by Automated.Multiple Development System 2 (AMD 2)
Application of thin layer
chromatography to profiling
3,4-methylenedioxymethamphetamine (MDMA).Zakrzewska1, J. Kochana1, A. Parczewski1,2,
Separation and detection of
Ergosterol and related Stereoids using temperature controlled planar
chromatography Planar chromatography in European Pharmacopeia, P. K. Zarzycki, M. B. Zarzycka
Optimization of the Immunodetection in planar chromatography.R. Akkad, G. Morlock, W. Schwack
New validated method for determination of acrylamide in potato chips by planar chromatography Müller, D.,
HPTLC/DART TOFT compared HPLC-ESI MS. G. Morlock
Possibilities and
limitations of the employment of y-spectroscopy in planar chromatography. Blagodarov, V., Morlock, G., Schwack, W
Thin-Layer Chromatography of lipid fraction in tree nuts species. Gerard Pujol,Josep M. Orellana, Nicolas Rozés, Joan R. Duran,Antoni Romeu. University Rovira i Virgili.43007 Tarragona Spain.
Quantification of pigments Astaxanthin and its Isomers by HPTLC in salmon fish feed . Vega H. Mario, Aranda B. Mario, Vega M. Mario, Capdeville Claudio, Villegas Ricardo, Muñoz Katherine, Bastías Corina and Torres Olga
HPTLC as Tool for Quality control in salmon culture. Evaluation of nutritive quality in salmon feed.Vega H. Mario, Aranda B. Mario, Vega M. Mario, Capdeville Claudio,Villegas Ricardo, Muñoz Katherine, Bastías Corina and
Simultaneous Determination of vitamin B2, vitamin B6, Niacin, caffein and Taurin by HPTLC/UV/FLD and HPTLC/ES-MS. M. Aranda, G. Morlock
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